1*Preeti Singh, 2 Sangeeta Singh
A B S T R A C T
The study was based on the Pharmacognostic and Anti-Stress Evaluation of Aqueous and Ethanolic Leaves Extract of Malvastrum coromandelianum. The fresh leaves of M. coromandelianum were collected from Unnao region and authenticated by the botanist at BSI, Prayagraj (Ref. no. 2024-25/549). The leaves were washed making dust-free and dried at room temperature or shade. The dried leaves were rendered into coarse powders and then finally into fine ones. The powder was weighed and soaked into ethanol and distilled water, separately for fifteen days with gradual stirrings. The obtained slurry of mixture is dried under partial vacuum using a rotary evaporator. Preliminary phytochemical screening was done. It was evaluated the anti-stress activity by following screening models i.e., Neuroprotective properties of aqueous and ethanolic extract of M. Coromandelianum against H2O2-Induced Cytotoxicity in SH-SY5Y Cells and Effect of M. Coromandelianum on HSP47-transformed cell viability models. The percentage yield of M. coromandelianum leaves extract was calculated as 43.28% and 52.54% in aqueous and ethanolic extract, respectively. In conclusion, aqueous and ethanolic leaf extract of Malvastrum Coromandelianum exhibited the anti-stress potential at different concentrations used in the study. In both the evaluation parameters i.e., Neuroprotective effect against H2O2-Induced Cytotoxicity in SH-SY5Y Cells and Cell viability/Stress effect using HSP47 Assay. Thus, it might be utilized in the management of stress; it’s anti-stress role may be due to presence of flavonoids, saponins etc. This study also suggests to identify the active constituents which is responsible for the anti-stress activity.
Keywords: Malvastrum coromandelianum, anti-stress, HSP47 Assay, H2O2-Induced Cytotoxicity.