1 *Mohit Kumar Soni, 2 Shalini Singh
A B S T R A C T
The study was based on evaluation of phytochemicals and anti-arthritic potential of methanolic leaves extract of Phyllanthus niruri. Fresh leaves of Phyllanthus niruri were obtained from the Unnao region. These were identified and authenticated by a botanist at BSI, Prayagraj (ref. 2024-25/543). Leaves were washed and dried in shade. The powder was weighed and soaked into methanol (95%) for 15 days with gradual stirrings. The obtained slurry of mixture was dried under partial vacuum using a rotary evaporator. Extraction the plant was done using methanol (95%) through maceration. Evaluation of the anti-arthritis activity of the methanolic herbal extract was performed through Inhibition of protein denaturation using bovine serum albumin and Inhibition of protein denaturation using egg albumin. The results showed that the methanolic leaves extract of P. niruri demonstrated the % inhibitionddddddddd of protein denaturation using BSA as 87.2±0.1 % and 92.5±0.4 % at the conc. of 400 µg/ml and 800 µg/ml respectively. It concluded that the anti-arthritic activity was found in methanolic leaves extract of P. niruri. On the other hand, a dose-dependent biological response was discovered. Furthermore, in both models, the extract’s aqueous fraction demonstrated a higher percent suppression of protein denaturation. The mechanism of action by which methanolic leaves extract of P. niruri treat and stop the progression of rheumatoid arthritis will be assessed by fellow researchers.
Keywords: Phyllanthus niruri, egg albumin, anti-arthritic, protein denaturation.