1 *Husain Asgar Khan, 2Kisalaya Mishra
A B S T R A C T
The current research was based on the pharmacological evaluation of crassula ovata of antinociceptive activity in albino rat. The fresh leaves of Crassula ovata were collected from Uttar Pradesh region and authenticated from a botanist with ref. no. CIMAP/Bot-Pharm/2021/20. The leaves were washed making dust-free and dried at room temperature or shade. The dried leaves were rendered into coarse powders and then finally into fine ones. The powder is weighed and soaked into ethanol solvent for 15 days with gradual stirrings (maceration process). The herbal extract was evaluated for their anti-nociceptive activity using hot-plate and tail-flick methods. Upon calculation, the percentage yield of ethanolic leaves extract of Crassula ovata was found as 63.58%. It showed phenols, flavonoids and terpenoids in moderate amount. However, phytosterol, steroid, carbohydrates, and proteins were found positive in C. ovata leaves extract. Saponins, While, saponins, tannins, glycoside, and plobatanin and starch were found absent. In results, ethanolic leaves extract of Crassula ovata were shown anti-nociceptive or analgesic potential when tested at 200mg/kg &400mg/kg. They exhibited dose-dependent activity and markedly increased the basal reaction time at various time intervals. In conclusion, inhibition of inflammatory mediators such as interleukins, thromboxane, cytokines, etc. may be the cause of analgesic activity. It refers to determine the mode of action that how ethanolic leaves extract of Crassula ovata subside pain whether its internal or external one.
Keywords: Crassula ovata, ethanolic extract, analgesic activity, hot plate method, tail-flick method