1 *Raman Srivastava, 2 Shalini Singh
A B S T R A C T
The study was based on the phytochemical screening of Extract Passiflora Vitifolia as an AntiDiabetic. Fresh leaves of Passiflora vitifolia were gathered from the Unnao region and verified by a botanist with ref. no. NIScPR/RHMD/Consult/2025/4486-87. The powdered leaves of Passiflora vitifolia was weighed then soaked into ethyl alcohol, methanol and water, separately for 15 days with gradual stirrings. The obtained slurry of mixture was filtered with filter paper and finally with Whatman filter paper. The obtained extract was made concentrated under partial vacuum using a rotary evaporator. Preliminary phytochemical screening and chromatographic analysis (HPLC, GC-MS) of Passiflora vitifolia extract were performed. Evaluation of antioxidant and antidiabetic potential of above extract through following screening models i.e., Estimation of DPPH Radical Scavenging Capacity, Estimation of α-amylase and Glucose uptake assay. In GC-MS interpretations, 7 compounds were shown much significant with wider absorption peaks. The compounds which were isolated from the ethanolic leaves extract of Passiflora vitifolia includes Styrene, 1-Dodecene, Palmitic acid, Linolelaidic acid, Heptadecane, 15-Hydroxypentadecanoic acid and acetic acid. The α-Amylase inhibition was observed as 59.51±0.27 %, 93.56±0.24 % and 43.32±0.56 % in methanolic leaves extract of Passiflora vitifolia, acarbose and water, respectively at the highest conc. (800µg/ml). It concluded that Passiflora vitifolia is an effective herb in the management of diabetes but it’s mode of action is still unknown. Further research may carried-out to confirm its mode of action and optimum doses required in management of diabetes mellitus.
Keywords: Passiflora vitifolia, anti-diabetic, α-Amylase inhibition, Palmitic acid.