1*Shubham Mitra, 2Shalini Singh
A B S T R A C T
The study was based on Phytochemical screening and evaluation of antioxidant and anti
arthritic potential of Crassula ovata leaves extract. The fresh leaves of Crassula ovata were
collected from the Unnao region in Uttar Pradesh. The plant herbarium will be authenticated by
a botanist. The leaves are washed for making dust-free dried under shade, sieved, and dried at
room temperature or shade. A 50g leaves powder of Crassula ovata was weighed and extracted
through cold maceration process i.e., soaked into beaker (mounted with aluminium foil) of
water: ethanol (1:) for 15 days with gradual stirrings. DPPH radical scavenging activity was
performed. Evaluation of the anti-arthritic potential of herbal extract was done through models
i.e., Inhibition of protein denaturation using bovine serum albumin and Inhibition of protein
denaturation using egg albumin. In results, the hydroalcoholic C. ovata leaves extract
demonstrated the % inhibition of protein denaturation using BSA as 57.3±0.4 % and 93.6±0.2 % at
the conc. of 12.5 µg/ml and 800 µg/ml respectively. Nonetheless, the C. ovata leaves extract
demonstrated the % inhibition of protein denaturation using egg albumin as 42.4±0.1 % and
92.6±0.2 % at the conc. of 12.5 µg/ml and 800 µg/ml respectively. In conclusion, C. ovata
leaves possess the potent antioxidant and anti-arthritic activity. On the other hand, a dose
dependent pharmacological response was observed.
Keywords: Crassula ovata, DPPH, anti-arthritic, BSA.